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Muscle Nerve. 2018 Sep;58(3):456-459. doi: 10.1002/mus.26140. Epub 2018 May 17.

Label-free identification of myopathological features with coherent anti-Stokes Raman scattering.

Author information

1
Department of Biophysics, Ruhr-University Bochum, Bochum, Germany.
2
Neurological Clinic Bergmannsheil, Ruhr-University Bochum, Bochum, Germany.
3
Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany.

Abstract

INTRODUCTION:

The aim of this study was the label-free identification of distinct myopathological features with coherent anti-Stokes Raman scattering (CARS) imaging, which leaves the sample intact for further analysis.

METHODS:

The protein distribution was determined without labels by CARS at 2,930 cm-1 and was compared with the results of standard histological staining.

RESULTS:

CARS imaging allowed the visualization of glycogen accumulation in glycogen storage disease type 5 (McArdle disease) and of internal nuclei in centronuclear myopathy. CARS identified an inhomogeneous protein distribution within muscle fibers in sporadic inclusion body myositis that was not shown with standard staining. In Duchenne muscular dystrophy, evidence for a higher protein content at the border of hypercontracted fibers was detected.

DISCUSSION:

CARS enables the label-free identification of distinct myopathological features, possibly paving the way for subsequent proteomic, metabolic, and genomic analyses. Muscle Nerve 58: 457-460, 2018.

KEYWORDS:

CARS; Raman imaging; histopathology; muscle myopathy; skeletal muscle

PMID:
29663456
DOI:
10.1002/mus.26140
[Indexed for MEDLINE]

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