Send to

Choose Destination
J Forensic Dent Sci. 2017 Sep-Dec;9(3):144-148. doi: 10.4103/jfo.jfds_39_16.

Age estimation using exfoliative cytology and radiovisiography: A comparative study.

Author information

Department of Oral Pathology, Mamata Dental College, Khammam, Telangana, India.



Age estimation is one of the essential factors in establishing the identity of an individual. Among various methods, exfoliative cytology (EC) is a unique, noninvasive technique, involving simple, and pain-free collection of intact cells from the oral cavity for microscopic examination.


The study was undertaken with an aim to estimate the age of an individual from the average cell size of their buccal smears calculated using image analysis morphometric software and the pulp-tooth area ratio in mandibular canine of the same individual using radiovisiography (RVG).

Materials and Methods:

Buccal smears were collected from 100 apparently healthy individuals. After fixation in 95% alcohol, the smears were stained using Papanicolaou stain. The average cell size was measured using image analysis software (Image-Pro Insight 8.0). The RVG images of mandibular canines were obtained, pulp and tooth areas were traced using AutoCAD 2010 software, and area ratio was calculated. The estimated age was then calculated using regression analysis.


The paired t-test between chronological age and estimated age by cell size and pulp-tooth area ratio was statistically nonsignificant (P > 0.05).


In the present study, age estimated by pulp-tooth area ratio and EC yielded good results.


Age estimation; exfoliative cytology; pulp–tooth area ratio; radiovisiography

Supplemental Content

Full text links

Icon for Medknow Publications and Media Pvt Ltd Icon for PubMed Central
Loading ...
Support Center