Efficient biocatalytic synthesis of nicotinic acid by recombinant nitrilase via high density culture

Jin-Song Gong; Qiang Zhang; Bing-Chen Gu; Ting-Ting Dong; Heng Li; Hui Li; Zhen-Ming Lu; Jin-Song Shi; Zheng-Hong Xu

doi:10.1016/j.biortech.2018.03.109

Efficient biocatalytic synthesis of nicotinic acid by recombinant nitrilase via high density culture

Bioresour Technol. 2018 Jul:260:427-431. doi: 10.1016/j.biortech.2018.03.109. Epub 2018 Mar 30.

Authors

Jin-Song Gong¹, Qiang Zhang¹, Bing-Chen Gu¹, Ting-Ting Dong¹, Heng Li¹, Hui Li¹, Zhen-Ming Lu², Jin-Song Shi³, Zheng-Hong Xu⁴

Affiliations

¹ Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, PR China.
² Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, PR China; National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
³ Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, PR China. Electronic address: shijs@163.com.
⁴ Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, PR China; National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China. Electronic address: zhenghxu@jiangnan.edu.cn.

PMID: 29655898
DOI: 10.1016/j.biortech.2018.03.109

Abstract

The constitutively expression system for P. putida nitrilase was firstly constructed to improve the nicotinic acid production and reduce the production costs. High density culture strategy was employed to enhance the biomass and nitrilase production of recombinant strain. The total nitrilase activity reached up to 654 U·mL^-1 without the induction. 541 g·L^-1 nicotinic acid was accumulated via fed batch mode of substrate feeding through 290 min of conversion.

Keywords: Biocatalysis; Fed-batch conversion; High density culture; Nicotinic acid; Nitrilase.

MeSH terms

Aminohydrolases*
Biocatalysis
Niacin

Substances

Niacin
Aminohydrolases
nitrilase