Procyanidin from peanut skin induces antiproliferative effect in human prostate carcinoma cells DU145

Chem Biol Interact. 2018 May 25:288:12-23. doi: 10.1016/j.cbi.2018.04.008. Epub 2018 Apr 11.

Abstract

In this study, the antiproliferative activity of peanut skin procyanidins (PSP) and six fractions (PSP-1∼6) isolated from PSP by several chromatographic steps on the human prostate cancer DU145 cells were evaluated. The results showed that PSP and PSP-1∼6 significantly inhibited the proliferation of DU145 cells. PSP-2 was the most effective fraction, which was identified as procyanidin B3 mainly and procyanidin dimer [(E)C-luteolin or keampferol] secondarily. Moreover, the mechanism of antiproliferative activity of PSP-2 was investigated. It was observed that PSP-2 induced apoptotic cell death and cell cycle arrest at S phase in DU145 cells. PSP-2 caused the increase of intracellular ROS level and the decrease of Bcl-2/Bax ratio, and triggered the activation of p53 and caspases-3 in DU145 cells. Our findings demonstrated that procyanidins from peanut skin have the potential to be developed as an anti-prostate cancer agent.

Keywords: Apoptosis; DU145; Peanut skin; Procyanidins; Prostate cancer.

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Apoptosis / drug effects
  • Arachis / chemistry*
  • Arachis / metabolism
  • Biflavonoids / chemistry*
  • Biflavonoids / isolation & purification
  • Biflavonoids / pharmacology
  • Caspase 3 / genetics
  • Caspase 3 / metabolism
  • Catechin / chemistry*
  • Catechin / isolation & purification
  • Catechin / pharmacology
  • Cell Cycle Checkpoints / drug effects
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Chromatography, High Pressure Liquid
  • Humans
  • Male
  • Microscopy, Electron, Transmission
  • Proanthocyanidins / chemistry*
  • Proanthocyanidins / isolation & purification
  • Proanthocyanidins / pharmacology
  • Prostatic Neoplasms / metabolism
  • Prostatic Neoplasms / pathology
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • Reactive Oxygen Species / metabolism
  • Tandem Mass Spectrometry
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / metabolism
  • bcl-2-Associated X Protein / genetics
  • bcl-2-Associated X Protein / metabolism

Substances

  • Antineoplastic Agents
  • Biflavonoids
  • Proanthocyanidins
  • Proto-Oncogene Proteins c-bcl-2
  • Reactive Oxygen Species
  • TP53 protein, human
  • Tumor Suppressor Protein p53
  • bcl-2-Associated X Protein
  • procyanidin
  • Catechin
  • Caspase 3