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Biochem Biophys Res Commun. 2018 Jun 2;500(2):325-332. doi: 10.1016/j.bbrc.2018.04.067. Epub 2018 Apr 13.

Regulation of hair follicle development by exosomes derived from dermal papilla cells.

Author information

1
Department of Dermatology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
2
Department of Dermatology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China. Electronic address: lzfskin@zju.edu.cn.

Abstract

BACKGROUND:

Dermal papilla cells (DPCs) play a critical role in the regulation of hair follicle (HF) growth, formation, and cycling. DPCs are thought to regulate HF growth through a paracrine mechanism, in which exosomes may play a critical role.

METHODS:

DPC-Exos were cutaneously injected into HFs at different HF cycle stages and the effects were evaluated by histological and immunohistochemical analyses. The effects of DPC-Exos on proliferation, migration, and cell cycle status of outer root sheath cells (ORSCs) were evaluated. After treatment of DPC-Exos, changes in mRNA and protein levels of β-catenin and Sonic hedgehog (Shh) in ORSCs were detected.

RESULTS:

DPC-Exos were approximately 105 nm in diameter and expressed tumor susceptibility gene 101, cluster of differentiation (CD)9, and CD63. Injection of DPC-Exos accelerated the onset of HF anagen and delayed catagen in mice. Immunohistochemical analyses revealed that β-catenin and Shh levels were upregulated in the skin. In vitro, DPC-Exo treatment enhanced ORSC proliferation and migration, and stimulated the expression of β-catenin and Shh.

CONCLUSION:

DPC-Exos contribute to the regulation of HF growth and development, and provide a potential avenue for the treatment of hair loss.

KEYWORDS:

Dermal papilla cell; Exosome; Hair follicle; Outer root sheath cell

PMID:
29654758
DOI:
10.1016/j.bbrc.2018.04.067
[Indexed for MEDLINE]

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