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Nanomaterials (Basel). 2018 Apr 13;8(4). pii: E235. doi: 10.3390/nano8040235.

Fabrication of Sericin/Agrose Gel Loaded Lysozyme and Its Potential in Wound Dressing Application.

Yang M1, Wang Y2,3, Tao G4, Cai R5, Wang P6, Liu L7, Ai L8, Zuo H9, Zhao P10,11, Umar A12, Mao C13,14, He H15,16.

Author information

1
State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China. yangmeirong@email.swu.edu.cn.
2
State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China. yjwang@swu.edu.cn.
3
College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China. yjwang@swu.edu.cn.
4
State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China. taogang@email.swu.edu.cn.
5
College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China. cairui0330@email.swu.edu.cn.
6
College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China. modelsums@email.swu.edu.cn.
7
State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China. l3341345@email.swu.edu.cn.
8
State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China. als123@email.swu.edu.cn.
9
College of Pharmaceutical Sciences, Southwest University, Beibei, Chongqing 400715, China. zuohua@swu.edu.cn.
10
State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China. zhaop@swu.edu.cn.
11
Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Beibei, Chongqing 400715, China. zhaop@swu.edu.cn.
12
Department of Chemistry, College of Science and Arts and Promising Centre for Sensors and Electronics Devices (PCSED), Najran University, P.O. Box 1988, Najran 11001, Saudi Arabia. umahmad@nu.edu.sa.
13
Department of Chemistry & Biochemistry, Stephenson Life Science Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019, USA. cbmao@ou.edu.
14
School of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China. cbmao@ou.edu.
15
State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China. hehuawei@swu.edu.cn.
16
Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Beibei, Chongqing 400715, China. hehuawei@swu.edu.cn.

Abstract

Sericin is a biomaterial resource for its significant biodegradability, biocompatibility, hydrophilicity, and reactivity. Designing a material with superabsorbent, antiseptic, and non-cytotoxic wound dressing properties is advantageous to reduce wound infection and promote wound healing. Herein, we propose an environment-friendly strategy to obtain an interpenetrating polymer network gel through blending sericin and agarose and freeze-drying. The physicochemical characterizations of the sericin/agarose gel including morphology, porosity, swelling behavior, crystallinity, secondary structure, and thermal property were well characterized. Subsequently, the lysozyme loaded sericin/agarose composite gel was successfully prepared by the solution impregnation method. To evaluate the potential of the lysozyme loaded sericin/agarose gel in wound dressing application, we analyzed the lysozyme loading and release, antimicrobial activity, and cytocompatibility of the resulting gel. The results showed the lysozyme loaded composite gel had high porosity, excellent water absorption property, and good antimicrobial activities against Escherichia coli and Staphylococcus aureus. Also, the lysozyme loaded gel showed excellent cytocompatibility on NIH3T3 and HEK293 cells. So, the lysozyme loaded sericin/agarose gel is a potential alternative biomaterial for wound dressing.

KEYWORDS:

agarose; composite gel; lysozyme; silk sericin; wound dressing

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