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Technol Cancer Res Treat. 2018 Jan 1;17:1533033818767936. doi: 10.1177/1533033818767936.

A Real-Time Near-Infrared Fluorescence Imaging Method for the Detection of Oral Cancers in Mice Using an Indocyanine Green-Labeled Podoplanin Antibody.

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1 Graduate School of Pharmaceutical Sciences, Meijo University, Nagoya, Japan.
2 Department of Pathology and Molecular Diagnostics, Aichi Cancer Center Central Hospital, Nagoya, Japan.
3 The First Department of Oral and Maxillofacial Surgery, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
4 Department of Oral and Maxillofacial Surgery, Daiyukai General Hospital, Ichinomiya, Japan.
5 Department of Regional Innovation, Tohoku University Graduate School of Medicine, Sendai, Japan.
6 Information and Communications Headquarters/Graduate School of Information Science, Nagoya University, Nagoya, Japan.
7 Department of Diagnostic Pathology, Daiyukai General Hospital, Ichinomiya, Japan.
8 Department of Clinical Oncology, Kyoto Pharmaceutical University, Kyoto, Japan.
9 Laboratory of Pathology and Clinical Research, Aichi Cancer Center Aichi Hospital, Okazaki, Japan.


Podoplanin is distinctively overexpressed in oral squamous cell carcinoma than oral benign neoplasms and plays a crucial role in the pathogenesis and metastasis of oral squamous cell carcinoma but its diagnostic application is quite limited. Here, we report a new near-infrared fluorescence imaging method using an indocyanine green (ICG)-labeled anti-podoplanin antibody and a desktop/a handheld ICG detection device for the visualization of oral squamous cell carcinoma-xenografted tumors in nude mice. Both near-infrared imaging methods using a desktop (in vivo imaging system: IVIS) and a handheld device (photodynamic eye: PDE) successfully detected oral squamous cell carcinoma tumors in nude mice in a podoplanin expression-dependent manner with comparable sensitivity. Of these 2 devices, only near-infrared imaging methods using a handheld device visualized oral squamous cell carcinoma xenografts in mice in real time. Furthermore, near-infrared imaging methods using the handheld device (PDE) could detect smaller podoplanin-positive oral squamous cell carcinoma tumors than a non-near-infrared, autofluorescence-based imaging method. Based on these results, a near-infrared imaging method using an ICG-labeled anti-podoplanin antibody and a handheld detection device (PDE) allows the sensitive, semiquantitative, and real-time imaging of oral squamous cell carcinoma tumors and therefore represents a useful tool for the detection and subsequent monitoring of malignant oral neoplasms in both preclinical and some clinical settings.


NZ-1 antibody; indocyanine green; near-infrared fluorescence imaging; oral squamous cell carcinoma; podoplanin

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