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Dev Comp Immunol. 2018 Aug;85:71-85. doi: 10.1016/j.dci.2018.04.007. Epub 2018 Apr 9.

The Florida manatee (Trichechus manatus latirostris) T cell receptor loci exhibit V subgroup synteny and chain-specific evolution.

Author information

1
Comparative Immunogenetics Laboratory, Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA. Electronic address: bbreaux@cvm.tamu.edu.
2
Sirenia Project, Wetland and Aquatic Research Center, U.S. Geological Survey, 7920 NW 71st Street, Gainesville, FL 32653, USA. Electronic address: mhunter@usgs.gov.
3
Laboratory of DNA Polymorphism, Federal University of Pará, Belém, Pará, Brazil. Electronic address: paula@ufpa.br.
4
Laboratory of Medical and Human Genetics, Federal University of Pará, Belém, Pará, Brazil. Electronic address: lsena@ufpa.br.
5
Sirenia Project, Wetland and Aquatic Research Center, U.S. Geological Survey, 7920 NW 71st Street, Gainesville, FL 32653, USA. Electronic address: rbonde@usgs.gov.
6
Comparative Immunogenetics Laboratory, Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA; Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M Health Science Center, Texas A&M University, College Station, TX 77843, USA. Electronic address: mcriscitiello@cvm.tamu.edu.

Abstract

The Florida manatee (Trichechus manatus latirostris) has limited diversity in the immunoglobulin heavy chain. We therefore investigated the antigen receptor loci of the other arm of the adaptive immune system: the T cell receptor. Manatees are the first species from Afrotheria, a basal eutherian superorder, to have an in-depth characterization of all T cell receptor loci. By annotating the genome and expressed transcripts, we found that each chain has distinct features that correlates to their individual functions. The genomic organization also plays a role in modulating sequence conservation between species. There were extensive V subgroup synteny blocks in the TRA and TRB loci between T. m. latirostris and human. Increased genomic locus complexity correlated to increased locus synteny. We also identified evidence for a VHD pseudogene for the first time in a eutherian mammal. These findings emphasize the value of including species within this basal eutherian radiation in comparative studies.

KEYWORDS:

Evolution; Manatee; Synteny blocks; T cell receptor; VHD

PMID:
29649552
DOI:
10.1016/j.dci.2018.04.007
[Indexed for MEDLINE]

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