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J Med Food. 2018 Jul;21(7):716-725. doi: 10.1089/jmf.2017.4116. Epub 2018 Mar 20.

Anti-Inflammatory Effects of a Mixture of Lactic Acid Bacteria and Sodium Butyrate in Atopic Dermatitis Murine Model.

Author information

1
1 Department of Animal Resources Technology, Gyeongnam National University of Science and Technology , Jinju, Korea.
2
2 Department of Animal Science, Chonnam National University , Gwangju, Korea.
3
3 Department of Biochemistry, Institute of Cell Differentiation and Aging, College of Medicine, Hallym University , Chuncheon, Korea.
4
4 Department of Nano-Bioengineering, Incheon National University , Incheon, Korea.
5
5 Department of Animal Science and Technology, Sunchon National University , Sunchon, Korea.
6
6 Department of Agricultural Biotechnology, Seoul National University , Seoul, Korea.
7
7 Department of Life Science, Silla University , Busan, Korea.

Abstract

Atopic dermatitis is a chronic and recurrent inflammatory skin disease. Recently, probiotics have been shown to suppress allergic symptoms through immunomodulatory responses. In the present study, combinatorial effects on allergic symptoms were identified in BALB/c mice fed with a mixture of four species of probiotics, Bifidobacterium lactis, Lactobacillus casei, Lactobacillus rhamnosus, and Lactobacillus plantarum, and sodium butyrate. Following sensitization with whey protein, the mice were challenged and divided into two groups: (1) mice administered with phosphate-buffered saline as a control and (2) mice administered with the probiotic mixture and sodium butyrate. Allergic symptoms were assessed by measuring ear thicknesses, serum histamine and IL-10 concentrations, and the quantities of leaked Evans blue. T cell differentiation was determined by analyzing the T cells groups in the mesenteric lymph nodes (MLNs) and spleen. To examine changes in the total gut microbiota, total fecal microflora was isolated, species identification was performed by DNA sequencing using Illumina MiSeq, and changes in intestinal beneficial bacteria were analyzed using quantitative polymerase chain reaction. Treatment with the probiotic mixture and sodium butyrate reduced ear thicknesses, the quantity of leaked Evans blue, and serum histamine values, while increasing serum IL-10 values. In the mouse model, the probiotic mixture and sodium butyrate increased Th1 and Treg cell differentiation in MLN and spleen tissues; the ratio of Firmicutes/Bacteroidetes, which is associated with reduction in allergic reactions; and microorganisms that lead to cell differentiation into Treg. These results suggest that the probiotic mixture and sodium butyrate can prevent and alleviate allergic symptoms.

KEYWORDS:

Galectin9; Th1 cells/Th2 cells; Treg cells; gut microbiota

PMID:
29648971
PMCID:
PMC6065516
[Available on 2019-07-01]
DOI:
10.1089/jmf.2017.4116
[Indexed for MEDLINE]

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