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J Leukoc Biol. 2018 Jul;104(1):123-133. doi: 10.1002/JLB.1MA1217-475R. Epub 2018 Apr 12.

Identification of a Siglec-F+ granulocyte-macrophage progenitor.

Author information

1
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
2
Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia.
3
Division of Allergy and Immunology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
4
School of Clinical Sciences at Monash Health, Monash University, Clayton, Victoria, Australia.

Abstract

In recent years multi-parameter flow cytometry has enabled identification of cells at major stages in myeloid development; from pluripotent hematopoietic stem cells, through populations with increasingly limited developmental potential (common myeloid progenitors and granulocyte-macrophage progenitors), to terminally differentiated mature cells. Myeloid progenitors are heterogeneous, and the surface markers that define transition states from progenitors to mature cells are poorly characterized. Siglec-F is a surface glycoprotein frequently used in combination with IL-5 receptor alpha (IL5Rα) for the identification of murine eosinophils. Here, we describe a CD11b+ Siglec-F+ IL5Rα- myeloid population in the bone marrow of C57BL/6 mice. The CD11b+ Siglec-F+ IL5Rα- cells are retained in eosinophil deficient PHIL mice, and are not expanded upon overexpression of IL-5, indicating that they are upstream or independent of the eosinophil lineage. We show these cells to have GMP-like developmental potential in vitro and in vivo, and to be transcriptionally distinct from the classically described GMP population. The CD11b+ Siglec-F+ IL5Rα- population expands in the bone marrow of Myb mutant mice, which is potentially due to negative transcriptional regulation of Siglec-F by Myb. Lastly, we show that the role of Siglec-F may be, at least in part, to regulate GMP viability.

KEYWORDS:

Myb; eosinophil; granulocyte; hematopoiesis; neutrophil

PMID:
29645346
PMCID:
PMC6320667
DOI:
10.1002/JLB.1MA1217-475R
[Indexed for MEDLINE]
Free PMC Article

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