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Hum Gene Ther. 2018 Aug;29(8):886-901. doi: 10.1089/hum.2017.220. Epub 2018 Jul 5.

High-Throughput Screening Identifies Kinase Inhibitors That Increase Dual Adeno-Associated Viral Vector Transduction In Vitro and in Mouse Retina.

Author information

1
1 Telethon Institute of Genetics and Medicine (TIGEM) , Pozzuoli, Italy .
2
2 Department of Precision Medicine, University of Campania Luigi Vanvitelli , Naples, Italy .
3
3 CEINGE Biotecnologie Avanzate, Naples, Italy .
4
4 Institute for Applied Mathematics "Mauro Picone," National Research Council , Naples, Italy .
5
5 Medical Genetics, Department of Advanced Biomedicine, Federico II University , Naples, Italy .

Abstract

Retinal gene therapy based on adeno-associated viral (AAV) vectors is safe and efficient in humans. The low intrinsic DNA transfer capacity of AAV has been expanded by dual vectors where a large expression cassette is split in two halves independently packaged in two AAV vectors. Dual AAV transduction efficiency, however, is greatly reduced compared to that obtained with a single vector. As AAV intracellular trafficking and processing are negatively affected by phosphorylation, this study set to identify kinase inhibitors that can increase dual AAV vector transduction. By high-throughput screening of a kinase inhibitors library, three compounds were identified that increase AAV transduction in vitro, one of which has a higher effect on dual than on single AAV vectors. Importantly, the transduction enhancement is exerted on various AAV serotypes and is not transgene dependent. As kinase inhibitors are promiscuous, siRNA-mediated silencing of targeted kinases was performed, and AURKA and B, PLK1, and PTK2 were among those involved in the increase of AAV transduction levels. The study shows that kinase inhibitor administration reduces AAV serotype 2 (AAV2) capsid phosphorylation and increases the activity of DNA-repair pathways involved in AAV DNA processing. Importantly, the kinase inhibitor PF-00562271 improves dual AAV8 transduction in photoreceptors following sub-retinal delivery in mice. The study identifies kinase inhibitors that increase dual and single AAV transduction by modulating AAV entry and post-entry steps.

KEYWORDS:

dual AAV; kinase inhibitors; retina

PMID:
29641320
PMCID:
PMC6098407
DOI:
10.1089/hum.2017.220
[Indexed for MEDLINE]
Free PMC Article

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