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Proteins. 2018 Jul;86(7):712-722. doi: 10.1002/prot.25505. Epub 2018 Apr 19.

Comparative proteome analysis reveals pathogen specific outer membrane proteins of Leptospira.

Author information

1
Regional Medical Research Centre (ICMR), Port Blair, Andaman and Nicobar Islands, 744101, India.
2
Department of Chemical Sciences, Ariel University, Ariel, 70400, Israel.
3
School of Life Sciences, Central University of Himachal Pradesh, Temporary Academic Block, Shahpur, District-Kangra, Himachal Pradesh, 176206, India.
4
Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Raebareli Road, Lucknow, Uttar Pradesh, 226025, India.

Abstract

Proteomes of pathogenic Leptospira interrogans and L. borgpetersenii and the saprophytic L. biflexa were filtered through computational tools to identify Outer Membrane Proteins (OMPs) that satisfy the required biophysical parameters for their presence on the outer membrane. A total of 133, 130, and 144 OMPs were identified in L. interrogans, L. borgpetersenii, and L. biflexa, respectively, which forms approximately 4% of proteomes. A holistic analysis of transporting and pathogenic characteristics of OMPs together with Clusters of Orthologous Groups (COGs) among the OMPs and their distribution across 3 species was made and put forward a set of 21 candidate OMPs specific to pathogenic leptospires. It is also found that proteins homologous to the candidate OMPs were also present in other pathogenic species of leptospires. Six OMPs from L. interrogans and 2 from L. borgpetersenii observed to have similar COGs while those were not found in any intermediate or saprophytic forms. These OMPs appears to have role in infection and pathogenesis and useful for anti-leptospiral strategies.

KEYWORDS:

Leptospira; beta barrel; clusters of orthologous groups; immunogenic; outer membrane protein; pathogenic; proteome

PMID:
29633350
DOI:
10.1002/prot.25505

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