In many biochip applications, it is advantageous to be able to immobilize biomolecules at specific locations on the surface of solid supports. In this protocol, we describe a photochemical surface patterning procedure based on thiol-ene/yne photochemistry which allows for the simple and rapid selective patterning of biomolecules on thiol-ene solid supports. We describe the preparation of solid supports which are required for the immobilization, including porous monoliths, as well as two different immobilization schemes based on biotin-streptavidin interactions and covalent linkage via free amino groups respectively.
Keywords: Amino groups; Biotin–streptavidin; Enzyme immobilization; Microfluidics; Monoliths; OSTE; Photochemistry; Photoimmobilization; Thiol–ene; Thiol–yne.