Format

Send to

Choose Destination
Genetics. 2018 Jun;209(2):537-549. doi: 10.1534/genetics.118.300839. Epub 2018 Apr 9.

Drosophila Glypicans Regulate Follicle Stem Cell Maintenance and Niche Competition.

Author information

1
Department of Genetics, Cell Biology and Development, The University of Minnesota, Minneapolis, Minnesota 55455.
2
Department of Genetics, Cell Biology and Development, The University of Minnesota, Minneapolis, Minnesota 55455 nakat003@umn.edu.

Abstract

Adult stem cells reside in specialized microenvironments called niches, which provide signals for stem cells to maintain their undifferentiated and self-renewing state. To maintain stem cell quality, several types of stem cells are known to be regularly replaced by progenitor cells through niche competition. However, the cellular and molecular bases for stem cell competition for niche occupancy are largely unknown. Here, we show that two Drosophila members of the glypican family of heparan sulfate proteoglycans (HSPGs), Dally and Dally-like (Dlp), differentially regulate follicle stem cell (FSC) maintenance and competitiveness for niche occupancy. Lineage analyses of glypican mutant FSC clones showed that dally is essential for normal FSC maintenance. In contrast, dlp is a hypercompetitive mutation: dlp mutant FSC progenitors often eventually occupy the entire epithelial sheet. RNA interference knockdown experiments showed that Dally and Dlp play both partially redundant and distinct roles in regulating Jak/Stat, Wg, and Hh signaling in FSCs. The Drosophila FSC system offers a powerful genetic model to study the mechanisms by which HSPGs exert specific functions in stem cell replacement and competition.

KEYWORDS:

Drosophila; follicle stem cells; glypicans; heparan sulfate; stem cell competition

PMID:
29632032
PMCID:
PMC5972425
[Available on 2019-06-01]
DOI:
10.1534/genetics.118.300839
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center