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J Conserv Dent. 2018 Jan-Feb;21(1):85-89. doi: 10.4103/JCD.JCD_126_17.

Scanning electron microscopic evaluation of marginal adaptation of AH-plus, GuttaFlow, and RealSeal at apical one-third of root canals - Part I: Dentin-sealer interface.

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Department of Conservative Dentistry and Endodontics, Dr. R. Ahmed Dental College and Hospital, Kolkata, West Bengal, India.



Complete seal at dentin-sealer-core interface of the root canal is required to maintain long-term periapical health.


The aim of this in vitro scanning electron microscopic (SEM) study was to determine which of the commonly used root canal sealer among AH-Plus, GuttaFlow, and RealSeal provides a superior marginal adaptation with the dentin in the apical third region of root canals.

Materials and Methods:

Selected 30 human freshly extracted maxillary central incisors were biomechanically prepared, then divided equally into three groups and obturated with AH-Plus, GuttaFlow, and RealSeal using single-cone obturation technique. After sectioning longitudinally, apical third of the roots was observed under SEM; dentin-sealer-core interface was focused. Marginal adaptation and interfacial gaps at dentin-sealer interface of all the samples were evaluated and analyzed statistically in this part of the article.

Statistical Analysis Used:

Analysis of variance and post hoc Tukey's test.


GuttaFlow exhibited least average marginal gap of 2.38 ± 1.43 μm, followed by AH-Plus (4.11 ± 2.85 μm) and RealSeal (4.65 ± 3.91 μm) with no significant difference (P > 0.05) among the groups.


As the dentin-sealer interfacial gap is seen to be minimum in GuttaFlow, it is better adapted to dentin in the apical third of root canals compared to AH-Plus and RealSeal.


AH-Plus; GuttaFlow; RealSeal; dentin-sealer interface; marginal adaptation; scanning electron microscope

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