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Biochim Biophys Acta Biomembr. 2018 Jun;1860(6):1403-1413. doi: 10.1016/j.bbamem.2018.03.030. Epub 2018 Apr 4.

Effect of dietary docosahexaenoic acid on rhodopsin content and packing in photoreceptor cell membranes.

Author information

1
Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, OH 44106, USA.
2
Research Service, Louis Stokes Cleveland Veterans Administration Medical Center, Cleveland, OH 44106, USA; Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
3
Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, OH 44106, USA. Electronic address: paul.park@case.edu.

Abstract

Docosahexaenoic acid (DHA) is enriched in photoreceptor cell membranes. DHA deficiency impairs vision due to photoreceptor cell dysfunction, which is caused, at least in part, by reduced activity of rhodopsin, the light receptor that initiates phototransduction. It is unclear how the depletion of membrane DHA impacts the structural properties of rhodopsin and, in turn, its activity. Atomic force microscopy (AFM) was used to assess the impact of DHA deficiency on membrane structure and rhodopsin organization. AFM revealed that signaling impairment in photoreceptor cells is independent of the oligomeric status of rhodopsin and causes adaptations in photoreceptor cells where the content and density of rhodopsin in the membrane is increased. Functional and structural changes caused by DHA deficiency were reversible.

KEYWORDS:

G protein-coupled receptor; Membrane protein; Phospholipid; Photoreceptor cell; Phototransduction; Retina

PMID:
29626443
PMCID:
PMC5912654
DOI:
10.1016/j.bbamem.2018.03.030
[Indexed for MEDLINE]
Free PMC Article

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