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Int J Nanomedicine. 2018 Mar 23;13:1841-1850. doi: 10.2147/IJN.S155475. eCollection 2018.

Synovitis in mice with inflammatory arthritis monitored with quantitative analysis of dynamic contrast-enhanced NIR fluorescence imaging using iRGD-targeted liposomes as fluorescence probes.

Wu H#1,2, Wu H#1,2, He Y1, Gan Z2, Xu Z1,2, Zhou M1,2, Liu S1,2, Liu H1.

Author information

1
Department of Ultrasonography, Guangdong Second Provincial General Hospital Affiliated to Southern Medical University, Guangzhou, China.
2
Department of Ultrasonography, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
#
Contributed equally

Abstract

Background:

Rheumatoid arthritis (RA) is a common inflammatory disorder characterized primarily by synovitis and pannus formation in multiple joints, causing joints destruction and irreversible disability in most cases. Early diagnosis and effective therapy monitoring of RA are of importance for achieving the favorable prognosis.

Methods:

We first prepared the targeted fluorescence probes, and then explored the feasibility of near-infrared (NIR) fluorescence molecular imaging to detect and evaluate the RA via the targeted fluorescence probes by quantitative analysis in this study.

Results:

The targeted fluorescence probes (indocyanine green-liposomes decorated with iRGD peptide [iLPs]) was successfully prepared. The quantitative analysis found that strong fluorescence signal was detected in inflamed paws and the fluorescence signal in iLPs group was 3.03-fold higher than that in non-targeted (indocyanine green-liposomes decorated without iRGD peptide [LPs]) group (P<0.01) at 15 min after injection, whereas the fluorescence signal from iLPs signal can almost not be observed in the non-inflamed paws, showing the high sensitivity and accuracy for arthritis by the NIR fluorescence imaging based on iLPs.

Conclusion:

The NIR fluorescence imaging by iLPs may facilitate improved arthritis diagnosis and early assessment of the disease progression by providing an in vivo characterization of angiogenesis in inflammatory joint diseases.

KEYWORDS:

diagnosis; iRGD-targeted probes; near-infrared fluorescence imaging; rheumatoid arthritis; synovitis

PMID:
29615837
PMCID:
PMC5870656
DOI:
10.2147/IJN.S155475
[Indexed for MEDLINE]
Free PMC Article

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