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Eur J Immunol. 1987 Dec;17(12):1843-6.

Molecular analysis of the antigen receptor of virus-specific cytotoxic T cells and identification of a new V alpha family.

Author information

1
Ontario Cancer Institute, Toronto, Canada.

Abstract

We have determined the primary structure of the alpha/beta-antigen receptor of a lymphocytic choriomeningitis virus (LCMV)-specific cytotoxic T cell clone (P14). The beta chain of the T cell receptor is composed of V beta 8.1, D beta and J beta 2.4 gene segments while the alpha chain uses a member of the V alpha 2 family and J alpha TA31. Southern blot analysis of a panel of six other independent LCMV-specific cytotoxic T cell clones did not reveal any preferential usage of the V alpha 2 or V beta 8 gene families in the cytotoxic LCMV response. Additionally, we have isolated and sequenced cDNA clones derived from the non-functionally rearranged alpha allele of P14. This transcript is composed of a unique V alpha gene segment which belongs to a new V alpha gene family.

PMID:
2961577
DOI:
10.1002/eji.1830171226
[Indexed for MEDLINE]

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