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Cancer Biomark. 2018;22(1):13-18. doi: 10.3233/CBM-170673.

Baicalein suppresses non small cell lung cancer cell proliferation, invasion and Notch signaling pathway.

Su G1,1, Chen H2,1, Sun X3.

Author information

1
Department of Thoracic Surgery, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong, China.
2
Department of Pharmacy, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China.
3
Department of Surgery, Boshan District Hospital of Traditional Chinese Medicine, Zibo 255200, Shandong, China.

Abstract

BACKGROUND:

Baicalein is an important Chinese herbal medicine and has multiple pharmacological activities. However, the biological mechanisms of the anti-tumor effects of Baicalein on non small cell lung cancer (NSCLC) still need to be understood.

METHODS:

Human NSCLC A549 and H1299 cells were pretreated with Baicalein or DMSO. Cells viability and transwell cell invasion assays were performed to assess cell proliferation and invasion. QRT-PCR assay was used to analyze mRNA expression levels of Twist1, E-cadhertin, Vimentin, Notch1 and hes-1. Western blot analysis was also performed to determine protein expression.

RESULTS:

In the study, we found that Baicalein had a significantly inhibited effect on proliferation ability of A549 and H1299 cells. Cells treated with Baicalein showed a down-regulated expression of CyclinD1 and CDK1 in A549 and H1299 cells. Furthermore, we found that Baicalein significantly inhibited cell invasion and Epithelial-Mesenchymal Transition (EMT) by up-regulating the mRNA and protein expression of E-cadherin and down-regulated the Twist1 and Vimentin expression, Moreover, Treatment of Baicalein down-regulated Notch1 and hes-1 expression in A549 and H1299 cells, which indicated that Baicalein could suppress the Notch signaling pathway.

CONCLUSION:

Our studies suggest that Baicalein may be a potential phytochemical flavonoid for therapeutics of NSCLC and serve as a molecular target for NSCLC.

KEYWORDS:

Baicalein; cell proliferation; epithelial-mesenchymal transition; non small cell lung cancer

PMID:
29614624
DOI:
10.3233/CBM-170673
[Indexed for MEDLINE]

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