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Biochem Biophys Res Commun. 1987 Nov 30;149(1):276-81.

An antibody to the receptor for insulin-like growth factor I inhibits the growth of MCF-7 cells in tissue culture.

Author information

1
Burroughs Wellcome Co., Research Triangle Park, N. C. 27709.

Abstract

Alpha IR-3, a monoclonal antibody to the insulin-like growth factor I receptor which blocks insulin-like growth factor I binding and inhibits its activity, inhibits the binding of 125I-insulin-like growth factor I to MCF-7 cells (an estrogen dependent human breast carcinoma cell line) with an IC-50 of approximately 100 ng/ml. It also inhibits the growth of MCF-7 cells cultured in 5% calf serum with approximately the same IC-50. Inhibition of growth occurs both when cells are cultured in the presence and absence of estrogen and is more pronounced when cells are grown at a low density. These findings demonstrate a requirement for insulin-like growth factor I for optimal growth of MCF-7 cells and suggest that it is an autocrine growth factor in these cells.

PMID:
2961338
DOI:
10.1016/0006-291x(87)91635-4
[Indexed for MEDLINE]

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