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New Phytol. 2018 Jul;219(1):422-435. doi: 10.1111/nph.15122. Epub 2018 Apr 3.

SIP1 participates in regulation of flowering time in rice by recruiting OsTrx1 to Ehd1.

Author information

1
CAS Center for Excellence in Molecular Plant Sciences, School of Life Sciences, University of Science & Technology of China, Hefei, Anhui, 230027, China.
2
School of Life Sciences, Anhui Agricultural University, Hefei, Anhui, 230036, China.
3
Key Laboratory of Rice Genetic Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, China.
4
State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310006, China.

Abstract

Flowering time (heading date) in rice (Oryza sativa) is an important agronomic trait that determines yield. The levels of histone H3 lysine 4 trimethylation (H3K4me3) modulated by TRITHORAX-like proteins regulate gene transcription, flowering time and environmental stress responses. However, plant TRITHORAX-like proteins have no known DNA-binding domain, and therefore the mechanism that gives sequence specificity to these proteins remains unclear. Here, we show that the rice TRITHORAX-like protein OsTrx1 is recruited to its target, Early heading date 1 (Ehd1), by the C2H2 zinc finger protein SDG723/OsTrx1/OsSET33 Interaction Protein 1 (SIP1). SIP1 binds to the promoter of Ehd1 and interacts with OsTrx1. Mutations in SIP1 led to a late heading date under long-day and short-day conditions. Defects in OsTrx1 or SIP1 led to reduced H3K4me3 levels at Ehd1, thus reducing Ehd1 expression. Together, our results show that the transcription factor SIP1 interacts with OxTrx1, allowing OsTrx1 to specifically target Ehd1, altering H3K4me3 levels, increasing Ehd1 expression and thereby promoting flowering.

KEYWORDS:

C2H2 zinc finger protein SIP1; H3K4me3; OsTrx1; flowering time; rice

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