Send to

Choose Destination
Adv Sci (Weinh). 2017 Dec 27;5(2):1700531. doi: 10.1002/advs.201700531. eCollection 2018 Feb.

Guidance and Self-Sorting of Active Swimmers: 3D Periodic Arrays Increase Persistence Length of Human Sperm Selecting for the Fittest.

Author information

Bio-Acoustic MEMS in Medicine (BAMM) LaboratoryCanary Center at Stanford for Cancer Early Detection Department of Radiology Stanford School of Medicine Stanford University Palo Alto CA 94304 USA.
Department of Physics Worcester Polytechnic Institute Worcester MA 01609 USA.
The Turek ClinicSan Francisco CA 94133 USA.
Department of OBGYN University of California San Francisco School of Medicine San Francisco CA 94158 USA.
Department of Obstetrics and Gynecology School of Medicine Stanford University Stanford CA 94305 USA.
Department of Electrical Engineering (by courtesy) Stanford University Stanford CA 94305 USA.


Male infertility is a reproductive disease, and existing clinical solutions for this condition often involve long and cumbersome sperm sorting methods, including preprocessing and centrifugation-based steps. These methods also fall short when sorting for sperm free of reactive oxygen species, DNA damage, and epigenetic aberrations. Although several microfluidic platforms exist, they suffer from structural complexities, i.e., pumps or chemoattractants, setting insurmountable barriers to clinical adoption. Inspired by the natural filter-like capabilities of the female reproductive tract for sperm selection, a model-driven design, featuring pillar arrays that efficiently and noninvasively isolate highly motile and morphologically normal sperm, with lower epigenetic global methylation, from raw semen, is presented. The Simple Periodic ARray for Trapping And isolatioN (SPARTAN) created here modulates the directional persistence of sperm, increasing the spatial separation between progressive and nonprogressive motile sperm populations within an unprecedentedly short 10 min assay time. With over 99% motility of sorted sperm, a 5-fold improvement in morphology, 3-fold increase in nuclear maturity, and 2-4-fold enhancement in DNA integrity, SPARTAN offers to standardize sperm selection while eliminating operator-to-operator variations, centrifugation, and flow. SPARTAN can also be applied in other areas, including conservation ecology, breeding of farm animals, and design of flagellar microrobots for diagnostics.


fertility; multiparticle collision dynamics; multiscale simulations; persistence length; sperm sorting

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center