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Forensic Sci Int Genet. 2018 Jul;35:9-13. doi: 10.1016/j.fsigen.2018.03.011. Epub 2018 Mar 27.

Sperm cell purification from mock forensic swabs using SOMAmer™ affinity reagents.

Author information

1
Somalogic, Inc., 2945 Wilderness Place, Boulder, CO 80301, United States.
2
Denver Police Department Crime Laboratory, 1371 Cherokee Street, Denver, CO 80204, United States.
3
Somalogic, Inc., 2945 Wilderness Place, Boulder, CO 80301, United States; Mackintosh Academy, 6717 S Boulder Road, Boulder, CO 80303, United States.
4
Somalogic, Inc., 2945 Wilderness Place, Boulder, CO 80301, United States; St. Andrew's School, 350 Noxontown Road, Middletown, DE 19709, United States.
5
Somalogic, Inc., 2945 Wilderness Place, Boulder, CO 80301, United States; The Scripps Research Institute, 10550 North Torrey Pines Rd, La Jolla, CA 92037, United States.
6
Denver Police Department Crime Laboratory, 1371 Cherokee Street, Denver, CO 80204, United States; Human Medical Genetics and Genomics Program, University of Colorado School of Medicine, Aurora, CO 80045, United States. Electronic address: greggory.laberge@denvergov.org.

Abstract

We have demonstrated a proof of concept with affinity-based purification of sperm cells from mock forensic samples using SOMAmer™ reagents, DNA-based affinity reagents developed by SomaLogic, Inc. SOMAmer reagents were selected in vitro using whole-cell SELEX to bind specifically with intact, detergent-treated sperm cells. Successful separation of sperm from epithelial cells and their debris was demonstrated using buccal swabs with added semen. Primarily male DNA profiles were generated from sperm cells eluted from the types of cotton swabs typically used for rape kit evidence collection. The quality of sperm DNA isolated from samples purified using SOMAmers is comparable to existing commercially available differential extraction-based methods at higher sperm concentrations. This purification method is simple, offers relatively rapid (<2hr) sperm purification, and can potentially be automated using robotic workstations. This work serves as proof of concept that demonstrates the first use of SOMAmer reagents as affinity ligands to bind sperm cells. With further development, this technique can potentially be used for high-throughput sexual assault forensic casework.

KEYWORDS:

Affinity reagents; Cell elution; DNA typing; Detergents; Forensic science; SELEX; SOMAmers; Sex offenses; Sperm purification

PMID:
29609058
DOI:
10.1016/j.fsigen.2018.03.011
[Indexed for MEDLINE]

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