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J Ethnopharmacol. 2018 Jun 28;220:57-66. doi: 10.1016/j.jep.2018.03.037. Epub 2018 Mar 31.

Artemisia asiatica ethanol extract exhibits anti-photoaging activity.

Author information

1
Department of Genetic Engineering and Biomedical Institute for convergence (BICS), Sungkyunkwan University, Suwon 16419, Republic of Korea.
2
Research and Business Foundation, Sungkyunkwan University, Suwon 16419, Republic of Korea.
3
Central Institue, BeautyCosmetic Co., Ltd., Eumseong 27414, Republic of Korea.
4
College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Republic of Korea. Electronic address: jhkim1@jbnu.ac.kr.
5
Department of Pharmaceutical Engineering, Cheongju University, Cheongju 28503, Republic of Korea. Electronic address: ysyi@cju.ac.kr.
6
Department of Genetic Engineering and Biomedical Institute for convergence (BICS), Sungkyunkwan University, Suwon 16419, Republic of Korea. Electronic address: jaecho@skku.edu.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Artemisia asiatica Nakai is a traditional herbal plant that has long been used in anti-inflammatory, anti-infective and skin protective remedies.

AIM OF THE STUDY:

In this study, traditionally known skin-protective activity of Artemisia asiatica Nakai was examined with its ethanol extract (Aa-EE) under various photoaging conditions using skin-originated cells, and the underlying mechanism was also examined using various types of cells.

MATERIALS AND METHODS:

Effects of Aa-EE on cell viability, photocytotoxicity, and expression of matrix metalloproteinases (MMPs), cyclooxygenase (COX)-2, and moisturizing factors were measured in B16F10, HEK293, NIH3T3, and HaCaT cells under untreated and ultraviolet B (UVB)-irradiation conditions. Anti-melanogenic effect of Aa-EE was also examined by measuring both melanin content in B16F10 cells and tyrosinase activity. Anti-photoaging mechanism of Aa-EE was explored by determining the activation levels of signaling molecules by immunoblotting analysis.

RESULTS:

Aa-EE protected HaCaT cells from UVB irradiation-induced death. Aa-EE increased the expression of a type 1 pro-collagen gene and decreased the expression of matrix metalloproteinases, and COX-2 in NIH3T3 cells induced by UVB. Aa-EE increased the expression of transglutamase-1, hyaluronic acid synthase (HAS)-2, and HAS-3 in HaCaT cells and decreased the production of melanin in α-melanocyte-stimulating hormone-stimulated B16F10 cells by suppressing tyrosinase activity and the expression of tyrosinase, microphthalmia-associated transcription factor, tyrosinase-related protein (TRP)-1 and TRP-2.

CONCLUSION:

The results suggest that Aa-EE could be skin-protective remedy with anti-photoaging, anti-apoptotic, skin remodeling, moisturizing, and anti-melanogenesis properties.

KEYWORDS:

Artemisia asiatica Nakai; Inflammation; Moisturizing; Photoaging; Skin remodeling; UVB

PMID:
29609010
DOI:
10.1016/j.jep.2018.03.037
[Indexed for MEDLINE]

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