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Monoclon Antib Immunodiagn Immunother. 2018 Jun;37(3):162-165. doi: 10.1089/mab.2018.0006. Epub 2018 Apr 2.

Epitope Mapping of Monoclonal Antibody PMab-48 Against Dog Podoplanin.

Author information

1
1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Sendai, Japan .
2
2 Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , Tokyo, Japan .
3
3 Department of Chemistry, Graduate School of Science, Chiba University , Chiba, Japan .
4
4 Division of Bioengineering, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo , Tokyo, Japan .
5
5 New Industry Creation Hatchery Center, Tohoku University , Sendai, Japan .

Abstract

Podoplanin (PDPN), a type I transmembrane sialoglycoprotein, is expressed on normal renal podocytes, pulmonary type I alveolar cells, and lymphatic endothelial cells. Increased expression of PDPN in cancers is associated with poor prognosis and hematogenous metastasis through interactions with C-type lectin-like receptor 2 (CLEC-2) on platelets. We previously reported a novel PMab-48 antibody, which is an anti-dog PDPN (dPDPN) monoclonal antibody (mAb) recognizing PDPN expressed in lymphatic endothelial cells. However, the binding epitope of PMab-48 is yet to be clarified. In this study, an enzyme-linked immunosorbent assay and flow cytometry were used to investigate epitopes of PMab-48. The results revealed that the critical epitope of PMab-48 comprises Asp29, Asp30, Ile31, Ile32, and Pro33 of dPDPN.

KEYWORDS:

dPDPN; dog podoplanin; epitope mapping; monoclonal antibody

PMID:
29608407
DOI:
10.1089/mab.2018.0006
[Indexed for MEDLINE]

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