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Structure. 2018 May 1;26(5):785-795.e4. doi: 10.1016/j.str.2018.03.004. Epub 2018 Mar 29.

Validating Resolution Revolution.

Author information

1
Department of Molecular Structural Biology, Institute of Microbiology & Genetics, GZMB, Georg-August-University Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany. Electronic address: pneuman2@uni-goettingen.de.
2
Department of Molecular Structural Biology, Institute of Microbiology & Genetics, GZMB, Georg-August-University Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.

Erratum in

Abstract

Recent advances in instrumentation and image-processing software have resulted in a resolution revolution in cryo-electron microscopy (cryo-EM) and a surge in the popularity of this technique. However, despite technical progress and hundreds of structures determined so far, development of standards assessing the agreement between the cryo-EM map and the respective model has fallen behind. Here we establish a validation procedure evaluating this agreement and applied it to a set of 565 cryo-EM structures. Analysis of the results revealed that three-quarters of the validated structures exhibit moderate or low agreement between the map and the corresponding model, mostly due to limited structural features possessed by these maps. Model re-refinement significantly improved the agreement for only one-fifth of the structures, reaffirming the necessity to re-evaluate map resolution. The presented procedure provides an approach to re-estimate the resolution of cryo-EM map areas interpreted by the model.

KEYWORDS:

FSC; FSC-based validation; RSCC-based validation; cryo-EM; estimation of resolution; quality criteria; validation

PMID:
29606592
DOI:
10.1016/j.str.2018.03.004
[Indexed for MEDLINE]
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