Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry

Methods Mol Biol. 2018:1764:133-151. doi: 10.1007/978-1-4939-7759-8_9.

Abstract

Native mass spectrometry (MS) enables the characterization of macromolecular assemblies with high sensitivity. It can reveal the stoichiometry of subunits as well as their two-dimensional interaction network and provide information regarding the dynamic behavior of macromolecular complexes. Here, we describe the workflow to perform native MS experiments. In addition, we illustrate the quality control analysis of proteins using MS in denaturing conditions.

Keywords: Macromolecular assemblies; Native mass spectrometry; Stoichiometry; Two-dimensional map of interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Histones / chemistry
  • Histones / metabolism*
  • Macromolecular Substances / chemistry
  • Macromolecular Substances / metabolism*
  • Mass Spectrometry / methods*
  • Ribonucleases / chemistry
  • Ribonucleases / metabolism*
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / metabolism*

Substances

  • Histones
  • Macromolecular Substances
  • Saccharomyces cerevisiae Proteins
  • Ribonucleases
  • POP2 protein, S cerevisiae