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Angew Chem Int Ed Engl. 2018 Jun 18;57(25):7369-7374. doi: 10.1002/anie.201713020. Epub 2018 Apr 26.

Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting.

Author information

1
Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 1350, Copenhagen, Denmark.
2
Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3b, 2200, Copenhagen, Denmark.
3
Disease Systems Biology Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3b, 2200, Copenhagen, Denmark.
4
Scientific Department, National Gallery London, Trafalgar Square, London, WC2N 5DN, UK.
5
Conservation Department, National Gallery London, Trafalgar Square, London, WC2N 5DN, UK.

Abstract

Ahead of display, a non-original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and duck egg white. Analysis of post-translational modifications detected several photo-oxidation products, which suggest that the egg experienced prolonged exposure to UV light and was likely applied long before the glue layer. Additionally, glycation products detected may indicate naturally occurring glycoprotein degradation or reaction with a carbohydrate material such as starch, identified by ATR-FTIR in a cross-section of a sample taken from the painting. Palaeoproteomics is shown to provide detailed characterisation of organic layers associated with mural paintings and therefore aids reconstruction of the conservation history of these objects.

KEYWORDS:

conservation science; cultural heritage; mass spectrometry; post-translational modifications; proteomics

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