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J Biol Chem. 2018 May 25;293(21):7930-7941. doi: 10.1074/jbc.RA118.001856. Epub 2018 Mar 30.

The plant defense signal galactinol is specifically used as a nutrient by the bacterial pathogen Agrobacterium fabrum.

Author information

1
From the UMR Ecologie Microbienne, CNRS, INRA, VetAgro Sup, UCBL, Université de Lyon, F-69622, Villeurbanne, Lyon, France and.
2
CNRS CEA Université Paris Sud, Université Paris-Saclay, Institute for Integrative Biology of the Cell (I2BC), Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.
3
From the UMR Ecologie Microbienne, CNRS, INRA, VetAgro Sup, UCBL, Université de Lyon, F-69622, Villeurbanne, Lyon, France and celine.lavire@univ-lyon1.fr.
4
CNRS CEA Université Paris Sud, Université Paris-Saclay, Institute for Integrative Biology of the Cell (I2BC), Avenue de la Terrasse, 91198 Gif-sur-Yvette, France solange.morera@i2bc.paris-saclay.fr.

Abstract

The bacterial plant pathogen Agrobacterium fabrum uses periplasmic-binding proteins (PBPs) along with ABC transporters to import a wide variety of plant molecules as nutrients. Nonetheless, how A. fabrum acquires plant metabolites is incompletely understood. Using genetic approaches and affinity measurements, we identified here the PBP MelB and its transporter as being responsible for the uptake of the raffinose family of oligosaccharides (RFO), which are the most widespread d-galactose-containing oligosaccharides in higher plants. We also found that the RFO precursor galactinol, recently described as a plant defense molecule, is imported into Agrobacterium via MelB with nanomolar range affinity. Structural analyses and binding mode comparisons of the X-ray structures of MelB in complex with raffinose, stachyose, galactinol, galactose, and melibiose (a raffinose degradation product) revealed how MelB recognizes the nonreducing end galactose common to all these ligands and that MelB has a strong preference for a two-unit sugar ligand. Of note, MelB conferred a competitive advantage to A. fabrum in colonizing the rhizosphere of tomato plants. Our integrative work highlights the structural and functional characteristics of melibiose and galactinol assimilation by A. fabrum, leading to a competitive advantage for these bacteria in the rhizosphere. We propose that the PBP MelB, which is highly conserved among both symbionts and pathogens from Rhizobiace family, is a major trait in these bacteria required for early steps of plant colonization.

KEYWORDS:

ABC transporter; Agrobacterium fabrum; RFOs; agrobacterium; bacteria; crystal structure; galactinol; microbiology; periplasmic binding protein; plant defense; sugar transport

PMID:
29602905
PMCID:
PMC5971467
DOI:
10.1074/jbc.RA118.001856
[Indexed for MEDLINE]
Free PMC Article

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