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J Biol Chem. 2018 Jul 6;293(27):10524-10535. doi: 10.1074/jbc.TM118.000372. Epub 2018 Mar 29.

Homologous recombination and the repair of DNA double-strand breaks.

Author information

1
From the Departments of Microbiology and Molecular Genetics and.
2
From the Departments of Microbiology and Molecular Genetics and wdheyer@ucdavis.edu.
3
Molecular and Cellular Biology, University of California, Davis, Davis, California 95616-8665.

Abstract

Homologous recombination enables the cell to access and copy intact DNA sequence information in trans, particularly to repair DNA damage affecting both strands of the double helix. Here, we discuss the DNA transactions and enzymatic activities required for this elegantly orchestrated process in the context of the repair of DNA double-strand breaks in somatic cells. This includes homology search, DNA strand invasion, repair DNA synthesis, and restoration of intact chromosomes. Aspects of DNA topology affecting individual steps are highlighted. Overall, recombination is a dynamic pathway with multiple metastable and reversible intermediates designed to achieve DNA repair with high fidelity.

KEYWORDS:

DNA damage; DNA endonuclease; DNA helicase; DNA polymerase; DNA recombination; DNA repair; DNA topoisomerase; DNA topology; genomic instability

PMID:
29599286
PMCID:
PMC6036207
DOI:
10.1074/jbc.TM118.000372
[Indexed for MEDLINE]
Free PMC Article

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