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Bioresour Technol. 2018 Jul;259:414-422. doi: 10.1016/j.biortech.2018.03.050. Epub 2018 Mar 15.

Metagenomic insight into methanogenic reactors promoting direct interspecies electron transfer via granular activated carbon.

Author information

1
Department of Civil, Environmental and Architectural Engineering, Korea University, Seoul 02841, South Korea.
2
Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, South Korea.
3
Department of Civil, Environmental and Architectural Engineering, Korea University, Seoul 02841, South Korea; KU-KIST Green School, Graduate School of Energy and Environment, Korea University, Seoul 02841, South Korea. Electronic address: heedeung@korea.ac.kr.

Abstract

To provide insight into direct interspecies electron transfer via granular activated carbon (GAC), the effect of GAC supplementation on anaerobic digestion was evaluated. Compared to control samples, the GAC supplementation increased the total amount of methane production and its production rate by 31% and 72%, respectively. 16S rDNA sequencing analysis revealed a shift in the archaeal community composition; the Methanosarcina proportion decreased 17%, while the Methanosaeta proportion increased 5.6%. Metagenomic analyses based on shotgun sequencing demonstrated that the abundance of pilA and omcS genes belonging to Geobacter species decreased 69.4% and 29.4%, respectively. Furthermore, the analyses suggested a carbon dioxide reduction pathway rather than an acetate decarboxylation pathway for methane formation. Taken together, these results suggest that GAC improved methane production performance by shifting the microbial community and altering functional genes associated with direct interspecies electron transfer via conductive materials.

KEYWORDS:

Anaerobic digestion; Direct interspecies electron transfer; Granular activated carbon; Metagenomics

PMID:
29597150
DOI:
10.1016/j.biortech.2018.03.050
[Indexed for MEDLINE]

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