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Nat Commun. 2018 Mar 28;9(1):1268. doi: 10.1038/s41467-018-03658-2.

Beclin1 restricts RNA virus infection in plants through suppression and degradation of the viral polymerase.

Li F1,2, Zhang C1,3, Li Y1,2, Wu G1,2, Hou X3, Zhou X4, Wang A5,6.

Author information

1
London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, N5V 4T3, Canada.
2
Department of Biology, Western University, London, ON, N6A 5B7, Canada.
3
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China.
4
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China.
5
London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, N5V 4T3, Canada. Aiming.Wang@AGR.GC.CA.
6
Department of Biology, Western University, London, ON, N6A 5B7, Canada. Aiming.Wang@AGR.GC.CA.

Abstract

Autophagy emerges as an essential immunity defense against intracellular pathogens. Here we report that turnip mosaic virus (TuMV) infection activates autophagy in plants and that Beclin1 (ATG6), a core component of autophagy, inhibits virus replication. Beclin1 interacts with NIb, the RNA-dependent RNA polymerase (RdRp) of TuMV, via the highly conserved GDD motif and the interaction complex is targeted for autophagic degradation likely through the adaptor protein ATG8a. Beclin1-mediated NIb degradation is inhibited by autophagy inhibitors. Deficiency of Beclin1 or ATG8a enhances NIb accumulation and promotes viral infection and vice versa. These data suggest that Beclin1 may be a selective autophagy receptor. Overexpression of a Beclin1 truncation mutant that binds to NIb but lacks the ability to mediate NIb degradation also inhibits virus replication. The Beclin1-RdRp interaction further extends to several RNA viruses. Thus Beclin1 restricts viral infection through suppression and also likely autophagic degradation of the viral RdRp.

PMID:
29593293
PMCID:
PMC5871769
DOI:
10.1038/s41467-018-03658-2
[Indexed for MEDLINE]
Free PMC Article

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