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Cell Rep. 2018 Mar 27;22(13):3385-3392. doi: 10.1016/j.celrep.2018.03.023.

The Murine Natural Cytotoxic Receptor NKp46/NCR1 Controls TRAIL Protein Expression in NK Cells and ILC1s.

Author information

1
Department of Life Sciences, Imperial College London, London SW7 2AZ, UK; Memorial Sloan Kettering Cancer Center, Zuckerman Research Center, 408 East 69th Street, New York, NY 10065, USA.
2
Immunology and Virology Program, Centre for Ophthalmology and Visual Science, The University of Western Australia, Crawley, Western Australia, Australia; Centre for Experimental Immunology, Lions Eye Institute, Nedlands, Western Australia, Australia.
3
Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.
4
Medical Research Center, Hammersmith Hospital, London W12 0NN, UK.
5
Department of Immunology, Max Rady College of Medicine, University of Manitoba, Winnipeg R3E 0T5, Manitoba, Canada.
6
Memorial Sloan Kettering Cancer Center, Zuckerman Research Center, 408 East 69th Street, New York, NY 10065, USA.
7
Department of Life Sciences, Imperial College London, London SW7 2AZ, UK. Electronic address: n.guerra@imperial.ac.uk.

Abstract

TRAIL is an apoptosis-inducing ligand constitutively expressed on liver-resident type 1 innate lymphoid cells (ILC1s) and a subset of natural killer (NK) cells, where it contributes to NK cell anti-tumor, anti-viral, and immunoregulatory functions. However, the intrinsic pathways involved in TRAIL expression in ILCs remain unclear. Here, we demonstrate that the murine natural cytotoxic receptor mNKp46/NCR1, expressed on ILC1s and NK cells, controls TRAIL protein expression. Using NKp46-deficient mice, we show that ILC1s lack constitutive expression of TRAIL protein and that NK cells activated in vitro and in vivo fail to upregulate cell surface TRAIL in the absence of NKp46. We show that NKp46 regulates TRAIL expression in a dose-dependent manner and that the reintroduction of NKp46 in mature NK cells deficient for NKp46 is sufficient to restore TRAIL surface expression. These studies uncover a link between NKp46 and TRAIL expression in ILCs with potential implications in pathologies involving NKp46-expressing cells.

KEYWORDS:

IL-15; IL-2; ILC1; NK cell; NKp46; TRAIL; natural killer cell

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