Format

Send to

Choose Destination
Epigenomics. 2018 Apr 1;10(4):395-408. doi: 10.2217/epi-2017-0122. Epub 2018 Mar 28.

Diet-dependent changes in the intestinal DNA methylome after introduction of enteral feeding in preterm pigs.

Author information

1
Comparative Pediatrics and Nutrition, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg DK 1870 C, Denmark.
2
Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, PR China.

Abstract

AIM:

To examine how enteral feeding affects the intestinal epigenome and gene expression just after preterm birth.

MATERIALS & METHODS:

Intestinal tissue from preterm pigs, modeling preterm infants, was collected at birth and 5 days after gradual introduction of infant formula or bovine colostrum. The intestinal tissue was analyzed by reduced representation bisulfite sequencing and real-time qPCR.

RESULTS:

Relative to colostrum, formula increased bacterial epithelial adherence and lipopolysaccharide binding protein (LBP) expression, which was regulated by promoter methylation. Diet-dependent changes in DNA methylation and/or mRNA expression were related to innate immune response, hypoxia, angiogenesis and epithelial-mesenchymal transition pathways (e.g., TTC38, IL8, C3, HIF1A and VEGFR1).

CONCLUSION:

Epigenetic changes may mediate important effects of the first feeding on intestinal development in preterm neonates.

KEYWORDS:

DNA methylation; epigenetic gene regulation; immunity; microbiota; nutrition; preterm birth

PMID:
29587528
DOI:
10.2217/epi-2017-0122
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center