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Viruses. 2018 Mar 27;10(4). pii: E152. doi: 10.3390/v10040152.

Identification of Ellagic Acid from Plant Rhodiola rosea L. as an Anti-Ebola Virus Entry Inhibitor.

Author information

1
College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China. user753951@163.com.
2
Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA. user753951@163.com.
3
College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China. ruikun7128@gmail.com.
4
Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA. ruikun7128@gmail.com.
5
Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio, TX 78227, USA. manantpadma@txbiomed.org.
6
Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA. aschaf5@uic.edu.
7
College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China. houlin5027@163.com.
8
College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China. tianjingzhen@163.com.
9
Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio, TX 78227, USA. rdavey@txbiomed.org.
10
Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA. hancheng@uic.edu.
11
Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA. lijun@uic.edu.

Abstract

The recent 2014-2016 West African Ebola virus epidemic underscores the need for the development of novel anti-Ebola therapeutics, due to the high mortality rates of Ebola virus infections and the lack of FDA-approved vaccine or therapy that is available for the prevention and treatment. Traditional Chinese medicines (TCMs) represent a huge reservoir of bioactive chemicals and many TCMs have been shown to have antiviral activities. 373 extracts from 128 TCMs were evaluated using a high throughput assay to screen for inhibitors of Ebola virus cell entry. Extract of Rhodiola rosea displayed specific and potent inhibition against cell entry of both Ebola virus and Marburg virus. In addition, twenty commercial compounds that were isolated from Rhodiola rosea were evaluated using the pseudotyped Ebola virus entry assay, and it was found that ellagic acid and gallic acid, which are two structurally related compounds, are the most effective ones. The activity of the extract and the two pure compounds were validated using infectious Ebola virus. The time-of-addition experiments suggest that, mechanistically, the Rhodiola rosea extract and the effective compounds act at an early step in the infection cycle following initial cell attachment, but prior to viral/cell membrane fusion. Our findings provide evidence that Rhodiola rosea has potent anti-filovirus properties that may be developed as a novel anti-Ebola treatment.

KEYWORDS:

Ebola; Marburg; Rhodiola rosea; Traditional Chinese medicine; ellagic acid; gallic acid

PMID:
29584652
PMCID:
PMC5923446
DOI:
10.3390/v10040152
[Indexed for MEDLINE]
Free PMC Article

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