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EBioMedicine. 2018 Apr;30:248-260. doi: 10.1016/j.ebiom.2018.03.010. Epub 2018 Mar 15.

Long Non-Coding RNAs Associated with Metabolic Traits in Human White Adipose Tissue.

Author information

1
Department of Biosciences and Nutrition, Novum, Floor 7, Karolinska Institutet, 141 86 Stockholm, Sweden. Electronic address: hui.gao@ki.se.
2
Department of Medicine, Karolinska Institutet, C2-94, Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden.
3
Department of Biosciences and Nutrition, Novum, Floor 7, Karolinska Institutet, 141 86 Stockholm, Sweden.
4
RIKEN Center for Life Science Technologies, Division of Genomic Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
5
Department of Medicine, Karolinska Institutet, C2-94, Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden. Electronic address: peter.arner@ki.se.

Abstract

Long non-coding RNAs (lncRNAs) belong to a recently discovered class of molecules proposed to regulate various cellular processes. Here, we systematically analyzed their expression in human subcutaneous white adipose tissue (WAT) and found that a limited set was differentially expressed in obesity and/or the insulin resistant state. Two lncRNAs herein termed adipocyte-specific metabolic related lncRNAs, ASMER-1 and ASMER-2 were enriched in adipocytes and regulated by both obesity and insulin resistance. Knockdown of either ASMER-1 or ASMER-2 by antisense oligonucleotides in in vitro differentiated human adipocytes revealed that both genes regulated adipogenesis, lipid mobilization and adiponectin secretion. The observed effects could be attributed to crosstalk between ASMERs and genes within the master regulatory pathways for adipocyte function including PPARG and INSR. Altogether, our data demonstrate that lncRNAs are modulators of the metabolic and secretory functions in human fat cells and provide an emerging link between WAT and common metabolic conditions.

KEYWORDS:

Adipocytes; Adiponectin; Lipolysis; Long non-coding RNAs; Metabolic traits; White adipose tissue

PMID:
29580841
PMCID:
PMC5952343
DOI:
10.1016/j.ebiom.2018.03.010
[Indexed for MEDLINE]
Free PMC Article

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