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Clin Res Hepatol Gastroenterol. 2018 Sep;42(4):347-352. doi: 10.1016/j.clinre.2017.12.001. Epub 2018 Mar 23.

Preoperative erythropoietin treatment improves survival following major hepatic resection in a cirrhotic rat model.

Author information

1
Department of Surgery, College of Medicine, Chung-Ang University, 224-1, Heuk Seok-Dong, Dongjak-Ku, Seoul, 156-755, South Korea.
2
Department of Surgery, College of Medicine, Chung-Ang University, 224-1, Heuk Seok-Dong, Dongjak-Ku, Seoul, 156-755, South Korea. Electronic address: choiys@cau.ac.kr.
3
Department of Pathology, College of Medicine, Soonchunhyang University, Chungnam, South Korea.
4
Department of Anesthesiology and Pain Medicine, College of Medicine, Chung-Ang University, Seoul, South Korea.
5
Department of Biomedical Engineering, School of Integrative Engineering, Chung-Ang University, Seoul, 156-756, South Korea.
6
Department of Laboratory Medicine, College of Medicine, Chung-Ang University, Seoul, South Korea.

Abstract

AIM:

Major hepatic resection of a cirrhotic liver may result in a fatal clinical course. Preoperative erythropoietin (EPO) treatment has been shown to have protective properties and to stimulate liver regeneration. This study aims to investigate the effect of preoperative EPO on survival following major hepatic resection in a cirrhotic rat model.

METHODS:

Cirrhotic liver was induced by intraperitoneal injection of thioacetamide (200mg/kg/mL) in 72 Lewis rats. Each 36 rats received EPO (1IU/g, every second day, 5 times preoperatively) or saline (control) and major hepatectomy (removal of the left and half of the median lobe) was performed. Biochemical and immunohistochemical parameters, cytokines and overall survival were compared following surgery.

RESULTS:

Rats that received preoperative EPO had decreased hepatic aspartate aminotransferase, alanine aminotransferase and interleukin (IL)-1β expression, 48hours following surgery. They had increased hepatocyte growth factor and vascular endothelial growth factor expression at 1hour, increased IL-6 expression at 24, 48 and 120hours and increased Ki-67, 120hours following surgery. Overall, survival was significantly improved among EPO-treated rats (P=0.034).

CONCLUSION:

Preoperative EPO treatment has a protective effect and stimulates liver regeneration, leading to improved overall survival following major hepatectomy in a cirrhotic rat model.

KEYWORDS:

Erythropoietin; Liver cirrhosis; Liver regeneration; Protective effect; Surgical resection

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