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J Agric Food Chem. 2018 Apr 11;66(14):3675-3682. doi: 10.1021/acs.jafc.8b00063. Epub 2018 Apr 2.

ACC Deaminase from Lysobacter gummosus OH17 Can Promote Root Growth in Oryza sativa Nipponbare Plants.

Author information

1
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences , Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology , Nanjing 210014 , Peoples Republic of China.

Abstract

Although Lysobacter species are a remarkable source of natural compounds with antibacterial and antifungal activities, the ability of these bacteria to produce plant growth promoters remains practically unknown. In this work, the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) has been isolated from the secretions of Lysobacter gummosus OH17, indicating the presence of an ACC deaminase, which was shown to be encoded in the gene peg_1256. The recombinant enzyme could not only deaminate ACC to provide 2-oxobutanoic acid but also catalyzed the amination of the 2-oxobutanoic acid, demonstrating, for the first time, that ACC deaminases can produce ACC. After the treatment of rice Oryza sativa Nipponbare plants with OH17 ACC deaminase, the ethylene production levels were 44% higher in comparison with the control experiments, allowing significant improvements in root, 10%, and stem, 14%, growth.

KEYWORDS:

ACC deaminase; Lysobacter; Oryza sativa Nipponbare; plant growth; rice growth promoter

PMID:
29578708
DOI:
10.1021/acs.jafc.8b00063
[Indexed for MEDLINE]

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