Format

Send to

Choose Destination
J Cell Biochem. 2018 Jul;119(7):5788-5797. doi: 10.1002/jcb.26759. Epub 2018 Mar 25.

The effect of combined pulsed wave low-level laser therapy and mesenchymal stem cell-conditioned medium on the healing of an infected wound with methicillin-resistant Staphylococcal aureus in diabetic rats.

Author information

1
Department of Biology and Anatomical Sciences, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
2
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4
Department of Medical Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5
Department of Medicine, Division of Hematology/Oncology, Rush University Medical Center, Chicago, Illinois.
6
Department of Immunology/Microbiology, Cancer Center, Chicago, Illinois.
7
Department of Pathology, The University of Chicago, Chicago, Illinois.
8
Pathology Department, Rush University Medical Center, Chicago, Illinois.
9
Department of Statistics, University of Qom, Qom, Iran.
10
School of medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
11
Noveratech LLC of Louisville, Louisville, Kentucky.
12
Department of Surgery, University of Louisville, Louisville, Kentucky.
13
Cellular and Molecular Biology Research Center, and Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Abstract

This study aims to investigate the combined effects of Pulsed wave low-level laser therapy (PW LLLT) and human bone marrow mesenchymal stem cell-conditioned medium (hBM-MSC-CM) on the microbial flora and tensiometrical properties of an infected wound model with methicillin-resistant staphylococcal aureus (MRSA) in an experimental model for Type 1 diabetes mellitus (TIDM). TIDM was induced in rats by streptozotocin (STZ). One full-thickness excision was made on the backs of the rats. Next, the rats were divided into the following groups: Group 1 was the control (placebo) group; Group 2 received hBM-MSCs-CM four times; Group 3 were laser PWLLLT (890 nm, 80 Hz, 0.2 J/cm2 ); and Group 4 received hBM-MSCs-CM +LASER. Wounds were infected with MRSA. Microbiological examinations were performed on days 4, 7, and 15. Tensiometerical examinations were carried out on the 15th day. One-way analysis of variance showed that laser and CM alone and/or in combination significantly increases the tensiomerical properties of the repaired wounds compared with control wounds. A combination of PW laser and CM was statistically more effective than other treated groups. Two-way analysis of variance showed that laser and CM alone and/or in combination significantly decreases the colony-forming units (CFUs) compared with the control group. The application of hBM-MSC-CM and PWlaser alone and/or together significantly accelerates the wound-healing process in MRSA-infected cutaneous wounds in TI DM in rats. Additionally, a combined application of hBM-MSC-CM and PWlaser demonstrates a synergistic effect on the wound-healing process in MRSA-infected cutaneous wounds in Type I DM rats.

KEYWORDS:

diabetes mellitus; diabetic foot ulcers; human bone marrow mesenchymal stem cell-conditioned medium; low-level laser therapy; methicillin-resistant staphylococcal aureus; microbial flora

PMID:
29574990
DOI:
10.1002/jcb.26759
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center