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Curr Cardiol Rep. 2018 Mar 24;20(5):35. doi: 10.1007/s11886-018-0979-6.

Light-Sheet Imaging to Elucidate Cardiovascular Injury and Repair.

Author information

1
Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095, USA.
2
Department of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
3
Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76010, USA.
4
Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095, USA. Thsiai@mednet.ucla.edu.
5
Department of Bioengineering, University of California, Los Angeles, CA, 90095, USA. Thsiai@mednet.ucla.edu.
6
Medical Engineering, California Institute of Technology, Pasadena, CA, 91106, USA. Thsiai@mednet.ucla.edu.

Abstract

PURPOSE OF REVIEW:

Real-time 3-dimensional (3-D) imaging of cardiovascular injury and regeneration remains challenging. We introduced a multi-scale imaging strategy that uses light-sheet illumination to enable applications of cardiovascular injury and repair in models ranging from zebrafish to rodent hearts.

RECENT FINDINGS:

Light-sheet imaging enables rapid data acquisition with high spatiotemporal resolution and with minimal photo-bleaching or photo-toxicity. We demonstrated the capacity of this novel light-sheet approach for scanning a region of interest with specific fluorescence contrast, thereby providing axial and temporal resolution at the cellular level without stitching image columns or pivoting illumination beams during one-time imaging. This cutting-edge imaging technique allows for elucidating the differentiation of stem cells in cardiac regeneration, providing an entry point to discover novel micro-circulation phenomenon with clinical significance for injury and repair. These findings demonstrate the multi-scale applications of this novel light-sheet imaging strategy to advance research in cardiovascular development and regeneration.

KEYWORDS:

Cardiovascular injury; Doxorubicin; Light-sheet imaging; Regeneration

PMID:
29574550
PMCID:
PMC5987244
DOI:
10.1007/s11886-018-0979-6
[Indexed for MEDLINE]
Free PMC Article

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