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J Biomed Mater Res A. 2018 Jul;106(7):1842-1850. doi: 10.1002/jbm.a.36384. Epub 2018 Mar 24.

Combination of blood and biphasic calcium phosphate microparticles for the reconstruction of large bone defects in dog: A pilot study.

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Université Côte d'Azur, CNRS, Inserm, iBV, France.
Centre Hospitalier Universitaire, Pôle de Chirurgie Réparatrice et Ostéo Articulaire, Nice, France.
CRIP, Centre de Recherche et d'Investigation Précliniques, ONIRIS, Nantes, France.
Graftys, Aix en Provence, France.
Centre Hospitalier Universitaire, Laboratoire central d'anatomopathologie, Nice, France.
Université de Nantes, CNRS, UMR 6230, CEISAM, Nantes, France.
Université de Nantes, Inserm UMRS 791, LIOAD, Nantes, France.


We previously reported that biphasic calcium phosphate (BCP) microparticles embedded in a blood clot induces ectopic bone formation in mice and repairs a critical femoral defect in rat. The present pilot study aimed to evaluate in dog and in two models of large defects the efficacy of this composite named "blood for reconstruction of bone" (BRB). We show here that BRB is a cohesive biomaterial easy to prepare from dog autologous blood and to mold to fill large bone defects. First in a model of cylindrical femoral condyle defect, the BRB was compared with BCP particles alone. After 8 weeks, this revealed that the amount of mature bone was slightly and significantly higher with BRB than with BCP particles. Second, in a model consisting in a 2 cm-long critical interruptive defect of the ulna, the BRB was compared with autologous bone. After 6 months, we observed that implantation of BRB can induce the complete reconstruction of the defect and that newly formed bone exhibits high regenerative potential. Comparison with the results obtained with autologous bone grafting strongly suggests that the BRB might be an efficient biomaterial to repair large bone defects, as an alternative or in addition to autologous bone.


autologous bone graft; biphasic calcium phosphate; blood; critical bone defects; dog


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