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Bioconjug Chem. 2018 Apr 18;29(4):1446-1453. doi: 10.1021/acs.bioconjchem.8b00128. Epub 2018 Mar 27.

A Fluorescent Probe for Stimulated Emission Depletion Super-Resolution Imaging of Vicinal-Dithiol-Proteins on Mitochondrial Membrane.

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Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering , Shenzhen University , Shenzhen , Guangdong 518060 , China.
Department of Chemistry , Korea University , Seoul 02841 , Korea.
Asan Medical Center, College of Medicine , University of Ulsan , Seoul 138-736 , Korea.
The School of East-West Medical Science , Kyung Hee University , Yongin , Gyeonggi-do 17104 , Korea.


Realizing the significant roles of vicinal-dithiol proteins (VDPs) in maintaining the cellular redox homeostasis and their implication in many diseases, we synthesized a smart arsenate based fluorescent probe 1 which can preferentially target the mitochondrial membrane-bound vicinal dithiol proteins (VDPs), especially voltage-dependent anion channel (VDAC2). The probe targetability was demonstrated by in vitro studies such as colocalization, stimulated emission depletion (STED) super-resolution imaging, proteomic MS/MS analysis, and Western blot analysis. The probe represents a rare example of fluorescence labeling of mitochondrial membrane-bound VDPs and can provide a new way to construct VDPs-specific fluorescent probes to gain deeper understanding of their roles in mitochondrial-related disorders.

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