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Brain Struct Funct. 2018 Jul;223(6):2673-2684. doi: 10.1007/s00429-018-1657-6. Epub 2018 Mar 22.

Dopamine receptors in the rat entopeduncular nucleus.

Author information

1
The Leslie and Susan Gonda Interdisciplinary Brain Research Center, Bar Ilan University, 52900, Ramat Gan, Israel.
2
The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, 52900, Ramat Gan, Israel.
3
The Paul Feder Laboratory on Alzheimer's Disease Research, Bar Ilan University, 52900, Ramat Gan, Israel.
4
The Leslie and Susan Gonda Interdisciplinary Brain Research Center, Bar Ilan University, 52900, Ramat Gan, Israel. alon.korngreen@biu.ac.il.
5
The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, 52900, Ramat Gan, Israel. alon.korngreen@biu.ac.il.

Abstract

Dopamine is critical for the normal functioning of the basal ganglia, modulating both input and output nuclei of this system. The distribution and function of each of the five dopamine receptor subtypes have been studied extensively in the striatum. However, the role of extrastriatal dopamine receptors in basal ganglia information processing is less clear. Here, we studied the anatomical distribution of dopamine receptors in one of the output nuclei of the rodent basal ganglia, the entopeduncular nucleus (EP). The presence of all dopamine receptor subtypes was verified in the EP using immunostaining. We detected co-localization of dopamine receptors with VGAT, which suggests presynaptic expression on GABAergic terminals. D1R and D2R were strongly colocalized with VGAT, whereas DR3-5 showed only sparse co-localization. We further labeled striatal or pallidal neurons with GFP and showed that only D1 receptors were co-localized with striatal terminals, while only D2R and D3R were co-localized with pallidal terminals. Dopamine receptors were also strongly co-localized with MAP2, indicating postsynaptic expression. Overall, these findings suggest that the dopaminergic system modulates activity in the EP both directly via postsynaptic receptors, and indirectly via GABAergic synapses stemming from the direct and indirect pathways.

KEYWORDS:

Basal ganglia; Dopamine receptors; Entopeduncular nucleus; Globus pallidus; Striatum

PMID:
29569008
DOI:
10.1007/s00429-018-1657-6
[Indexed for MEDLINE]

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