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Ecotoxicol Environ Saf. 2018 Jul 30;156:279-286. doi: 10.1016/j.ecoenv.2018.03.031. Epub 2018 Mar 20.

Ectopic expression of SaNRAMP3 from Sedum alfredii enhanced cadmium root-to-shoot transport in Brassica juncea.

Author information

1
MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China. Electronic address: yfeng@zju.edu.cn.
2
MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.

Abstract

SaNRAMP3 gene cloned from a Zn/Cd hyperaccumulator Sedum alfredii was ectopicly expressed in Brassica juncea, a fast-growing and high-biomass crop plant. In a tissue culture experiment, transgenic plants were grown on MS medium with 0, 25, 50, 100, 200 μM Cd. It was shown that, at the same Cd treatment, the Cd tolerance of transgenic plants had no significant difference with those of wild-type plants (WT). However, the shoot Cd content and accumulation were improved significantly while the root Cd content and accumulation were descended significantly by SaNRAMP3 gene expression, which obviously enhanced the Cd root-to-shoot translocation factor (TF). In the hydroponic experiment, plants were cultured in nutrition solution with 0, 2.5, 25 μM Cd. Data showed that the Cd tolerance of transgenic plants had no significant difference with that of WT under the same Cd exposure. Whereas, the shoot Cd content and accumulation was increased 1.43-1.81 times and the TF was enhanced 3.09-3.51 times by SaNRAMP3 gene expression. Those results indicated that ectopic expression of SaNRAMP3 in B. juncea didn't lead to Cd sensitivity, but enhanced Cd root-to-shoot transport, so that increased shoot Cd accumulation. This study provided a possibility to improve phytoextraction efficiency of heavy metal through gene engineering.

KEYWORDS:

Cd; Gene engineering; Heavy metal; Hyperaccumulator; Phytoextraction

PMID:
29567508
DOI:
10.1016/j.ecoenv.2018.03.031
[Indexed for MEDLINE]

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