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Biomed Pharmacother. 2018 Aug;104:832-840. doi: 10.1016/j.biopha.2018.03.013. Epub 2018 Mar 19.

MiR-422a targets MAPKK6 and regulates cell growth and apoptosis in colorectal cancer cells.

Author information

1
Department of Clinical Laboratory, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, China.
2
Department of Clinical Laboratory, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, China. Electronic address: yannqiang_zhang@126.com.

Abstract

The important role of miR-422a in tumor has been reported in several studies. Recent research discovered that the expression of miR-422a was significantly decreased in colorectal cancer tissues, providing miR-422a as a tumor suppressor in CRC. However, the concrete mechanism of miR-422a regulating CRC cell is still unclear. In this study, we demonstrated that miR-422a could inhibit CRC cell growth and promote cell apoptosis via in vitro analyses. Moreover, computational methods were adopted to identify the targets of miR-422a. We found MAPKK6 was the direct target of miR-422a. Consequently, we further elucidated that miR-422a inhibited CRC cell growth and induced cell apoptosis by inhibiting p38/MAPK pathway. Besides that, we established the tumor xenograft model using nude mice and the inhibitory effects on tumor volumes and weights by miR-422a mimic transfection were also detected. Taken together, these findings demonstrated miR-422a exerted anti-cancer activities on CRC, which could be potentially used for CRC prognosis prediction and treatment.

KEYWORDS:

Apoptosis; Cell growth; Colorectal cancer; miRNA; p38

PMID:
29566993
DOI:
10.1016/j.biopha.2018.03.013
[Indexed for MEDLINE]

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