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FASEB J. 2018 Sep;32(9):4776-4790. doi: 10.1096/fj.201800034R. Epub 2018 Mar 22.

A combination of lipidomics, MS imaging, and PET scan imaging reveals differences in cerebral activity in rat pups according to the lipid quality of infant formulas.

Author information

1
Centre de Recherche Cardiovasculaire et Nutritionnelle (C2VN), INSERM, Institut National de la Recherche Agricole (INRA), BioMet, Aix-Marseille University, Marseille, France.
2
Laboratoire de Neuroendocrinologie Moléculaire de la Prise Alimentaire (NMPA), Centre de Neurosciences Université Paris-Sud (CNPS), Orsay, France.
3
Lactalis Recherche et Développement, Retiers, France.
4
Centre Européen de Recherche en Imagerie Médicale (CERIMED), Université d'Aix-Marseille, Marseille, France.
5
Institut National de la Recherche Agricole (INRA), Physiopathologie des Adaptations Nutritionnelles (PHAN), Université de Nantes, Nantes, France, France.
6
Thermo Fisher Scientific, Courtaboeuf, France.
7
Food Nutrition and Health Team, Food and Bio-Based Products Group, AgResearch Grasslands Research Centre, Riddet Institute, Massey University, Palmerston North, New Zealand.

Abstract

We evaluated the effect of adding docosahexaenoic:arachidonic acids (3:2) (DHA+ARA) to 2 representative commercial infant formulas on brain activity and brain and eye lipids in an artificially reared rat pup model. The formula lipid background was either a pure plant oil blend, or dairy fat with a plant oil blend (1:1). Results at weaning were compared to breast milk-fed pups. Brain functional activity was determined by positron emission tomography scan imaging, the brain and eye fatty acid and lipid composition by targeted and untargeted lipidomics, and DHA brain regional location by mass-spectrometry imaging. The brain functional activity was normalized to controls with DHA+ARA added to the formulas. DHA in both brain and eyes was influenced by formula intake, but more than two-thirds of tissue DHA-glycerolipids remained insensitive to the dietary challenge. However, the DHA lipidome correlated better with brain function than sole DHA content ( r = 0.70 vs. r = 0.48; P < 0.05). Brain DHA regional distribution was more affected by the formula lipid background than the provision of PUFAs. Adding DHA+ARA to formulas alters the DHA content and lipidome of nervous tissue in the neonate, making it closer to dam milk-fed controls, and normalizes brain functional activity.-Aidoud, N., Delplanque, B., Baudry, C., Garcia, C., Moyon, A., Balasse, L., Guillet, B., Antona, C., Darmaun, D., Fraser, K., Ndiaye, S., Leruyet, P., Martin, J.-C. A combination of lipidomics, MS imaging, and PET scan imaging reveals differences in cerebral activity in rat pups according to the lipid quality of infant formulas.

KEYWORDS:

artificial rearing; lactation; neonatal nutrition; omics

PMID:
29565735
DOI:
10.1096/fj.201800034R

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