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Oncol Rep. 2018 May;39(5):2136-2146. doi: 10.3892/or.2018.6328. Epub 2018 Mar 20.

FAT1 inhibits cell migration and invasion by affecting cellular mechanical properties in esophageal squamous cell carcinoma.

Author information

1
Translational Medicine Research Center, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
2
Department of Otorhinolaryngology, Shanxi Provincial People's Hospital, Taiyuan, Shanxi 030001, P.R. China.
3
Department of Pharmacology, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.

Abstract

FAT atypical cadherin 1 (FAT1) belongs to the cadherin superfamily and has been reported to regulate cell‑cell adhesion and other cell behaviors, suggesting its pivotal roles in human cancers. We previously identified FAT1 as one of the significant mutant genes in esophageal squamous cell carcinoma (ESCC). In the present study, the knockdown of FAT1 expression in YSE2 and Colo680N cell lines was carried out by lentivirus, and we found that knockdown of FAT1 led to acceleration of cell migration and invasion. Furthermore, we detected the cell adhesive force and cell elasticity force by atomic force microscopy (AFM) and found that the suppression of endogenous expression of FAT1 led to a decrease in the cell adhesive force and increase in the cell elasticity force compared with the control groups. In conclusion, our study demonstrated that FAT1 altered cellular mechanical properties leading to deregulation of cell migration and invasion of ESCC, which may be a novel target for ESCC therapy.

PMID:
29565465
PMCID:
PMC5928768
DOI:
10.3892/or.2018.6328
[Indexed for MEDLINE]
Free PMC Article

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