Format

Send to

Choose Destination
Appl Microbiol Biotechnol. 2018 May;102(9):4131-4142. doi: 10.1007/s00253-018-8921-9. Epub 2018 Mar 21.

A 12-residue epitope displayed on phage T7 reacts strongly with antibodies against foot-and-mouth disease virus.

Author information

1
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
2
Laboratory of Vaccines and Immunotherapeutics, Institute of Biosciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
3
Malaysia Genome Institute, National Institutes of Biotechnology Malaysia, Ministry of Science, Technology and Innovation, 43000, Kajang, Selangor, Malaysia.
4
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
5
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. wstan@upm.edu.my.
6
Laboratory of Vaccines and Immunotherapeutics, Institute of Biosciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. wstan@upm.edu.my.

Abstract

Foot-and-mouth disease (FMD) is a major threat to the livestock industry worldwide. Despite constant surveillance and effective vaccination, the perpetual mutations of the foot-and-mouth disease virus (FMDV) pose a huge challenge to FMD diagnosis. The immunodominant region of the FMDV VP1 protein (residues 131-170) displayed on phage T7 has been used to detect anti-FMDV in bovine sera. In the present study, the functional epitope was further delineated using amino acid sequence alignment, homology modelling and phage display. Two highly conserved regions (VP1145-152 and VP1159-170) were identified among different FMDV serotypes. The coding regions of these two epitopes were fused separately to the T7 genome and displayed on the phage particles. Interestingly, chimeric phage displaying the VP1159-170 epitope demonstrated a higher antigenicity than that displaying the VP1131-170 epitope. By contrast, phage T7 displaying the VP1145-152 epitope did not react significantly with the anti-FMDV antibodies in vaccinated bovine sera. This study has successfully identified a smaller functional epitope, VP1159-170, located at the C-terminal end of the structural VP1 protein. The phage T7 displaying this shorter epitope is a promising diagnostic reagent to detect anti-FMDV antibodies in vaccinated animals.

KEYWORDS:

Diagnostic reagent; ELISA; Foot-and-mouth disease; Phage display; VP1 epitope

PMID:
29564523
DOI:
10.1007/s00253-018-8921-9
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center