Format

Send to

Choose Destination
Int J Mol Sci. 2018 Mar 21;19(4). pii: E929. doi: 10.3390/ijms19040929.

The Complete Chloroplast Genome Sequence of Tree of Heaven (Ailanthus altissima (Mill.) (Sapindales: Simaroubaceae), an Important Pantropical Tree.

Saina JK1,2,3,4, Li ZZ5,6, Gichira AW7,8,9, Liao YY10.

Author information

1
Fairy Lake Botanical Garden, Shenzhen & Chinese Academy of Sciences, Shenzhen 518004, China. jksaina@wbgcas.cn.
2
Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China. jksaina@wbgcas.cn.
3
University of Chinese Academy of Sciences, Beijing 100049, China. jksaina@wbgcas.cn.
4
Sino-African Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China. jksaina@wbgcas.cn.
5
Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China. wbg_georgelee@163.com.
6
University of Chinese Academy of Sciences, Beijing 100049, China. wbg_georgelee@163.com.
7
Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China. gichira@wbgcas.cn.
8
University of Chinese Academy of Sciences, Beijing 100049, China. gichira@wbgcas.cn.
9
Sino-African Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China. gichira@wbgcas.cn.
10
Fairy Lake Botanical Garden, Shenzhen & Chinese Academy of Sciences, Shenzhen 518004, China. liaoyiying666@163.com.

Abstract

Ailanthus altissima (Mill.) Swingle (Simaroubaceae) is a deciduous tree widely distributed throughout temperate regions in China, hence suitable for genetic diversity and evolutionary studies. Previous studies in A. altissima have mainly focused on its biological activities, genetic diversity and genetic structure. However, until now there is no published report regarding genome of this plant species or Simaroubaceae family. Therefore, in this paper, we first characterized A. altissima complete chloroplast genome sequence. The tree of heaven chloroplast genome was found to be a circular molecule 160,815 base pairs (bp) in size and possess a quadripartite structure. The A. altissima chloroplast genome contains 113 unique genes of which 79 and 30 are protein coding and transfer RNA (tRNA) genes respectively and also 4 ribosomal RNA genes (rRNA) with overall GC content of 37.6%. Microsatellite marker detection identified A/T mononucleotides as majority SSRs in all the seven analyzed genomes. Repeat analyses of seven Sapindales revealed a total of 49 repeats in A. altissima, Rhus chinensis, Dodonaea viscosa, Leitneria floridana, while Azadirachta indica, Boswellia sacra, and Citrus aurantiifolia had a total of 48 repeats. The phylogenetic analysis using protein coding genes revealed that A. altissima is a sister to Leitneria floridana and also suggested that Simaroubaceae is a sister to Rutaceae family. The genome information reported here could be further applied for evolution and invasion, population genetics, and molecular studies in this plant species and family.

KEYWORDS:

Ailanthus altissima; Sapindales; Simaroubaceae; chloroplast genome; microsatellites

PMID:
29561773
PMCID:
PMC5979363
DOI:
10.3390/ijms19040929
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center