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Environ Sci Process Impacts. 2018 Mar 21. doi: 10.1039/C7EM00522A. [Epub ahead of print]

A novel method for source-specific hemoglobin adducts of nitro-polycyclic aromatic hydrocarbons.

Author information

1
Columbia Center for Children's Environmental Health, Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, 722 W. 168th St., 12th Floor, New York, NY 10032, USA. fpp1@cumc.columbia.edu.
2
Nicholas School of the Environment, Duke Global Health Institute, Duke University, LSRC Room A309, 308 Research Drive, Durham, NC 27708, USA. junfeng.zhang@duke.edu.
3
Department of Preventive Medicine, Keck School of Medicine, University of Southern California, USA.
4
Department of Mental Health, Bloomberg School of Public Health, Johns Hopkins University, USA.
5
Columbia Center for Children's Environmental Health, Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, 722 W. 168th St., 12th Floor, New York, NY 10032, USA. fpp1@cumc.columbia.edu and Department of Biostatistics, Mailman School of Public Health, Columbia University, USA.
6
Department of Analytical, Environmental & Forensic Sciences, Environmental Toxicology Group, MRC-PHE Centre for Environment & Health, NIHR Health Protection Research Unit in Health Impact of Environmental Hazards, King's College London, UK.
7
Chemistry and Chemical Engineering Division, Southwest Research Institute, USA.
8
Nicholas School of the Environment, Duke Global Health Institute, Duke University, LSRC Room A309, 308 Research Drive, Durham, NC 27708, USA. junfeng.zhang@duke.edu and College of Environmental Sciences and Engineering & BIC-ESAT, Peking University, Beijing, China.

Abstract

Polycyclic aromatic hydrocarbons (PAH) are ubiquitous air pollutants associated with negative impacts on growth, development and behavior in children. Source-specific biological markers of PAH exposure are needed for targeting interventions to protect children. Nitro-derivatives of PAH can act as markers of exposure to diesel exhaust, gasoline exhaust, or general combustion sources. Using a novel HPLC-APCI-MS/MS detection method, we examined four hemoglobin (Hb) adducts of nitro-PAH metabolites and the Hb adduct of a benzo[a]pyrene (BaP) metabolite in 22 umbilical cord blood samples. The samples were collected from a birth cohort with comprehensive data on prenatal PAH exposure, including prenatal personal air monitoring and DNA adducts in maternal and umbilical cord blood. Using non-parametric analyses, heat maps, and principal component analysis (PCA), we analyzed the relationship between the five Hb adducts and previous PAH measurements, with each measurement representing a different duration of exposure. We found that Hb adducts derived from several diesel-related nitro-PAHs (2-nitrofluorene and 1-nitropyrene) were significantly correlated (r = 0.77, p ≤ 0.0001) and grouped together in PCA. Nitro-PAH derived Hb adducts were largely unrelated to previously collected measures of exposure to a number of PAH parent compounds. These measures need to be validated in a larger sample.

PMID:
29561551
PMCID:
PMC6150855
[Available on 2019-09-21]
DOI:
10.1039/C7EM00522A

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